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Total RNA was isolated from the frozen biopsies using TRIzol reagent (Invitrogen, Carlsbad, USA) and purified with the RNeasy Mini Kit (Qiagen, Venlo, the Netherlands)

2015 doi: 10.1038/nature14112

The number of toxins our body has to deal with every day is truly remarkable
Enhanced mitochondrial biogenesis contributes to Wnt induced osteoblastic differentiation of C3H10T1/2 cells