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Description
Conditioned medium was harvested, filtered through 0.45-m filters, concentrated by ultracentrifugation (81550 g, 2 h at 4 C) and stored at 80 C
Remember, cooling tower water is full of air, so air in the piping is a given
Acknowledgements We thank D
doi: 10.1056/NEJMoa2002032 25 NishigaMWangDWHanYLewisDBWuJC
