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(2000) Evidence for the role of megalin in renal uptake of transthyretin

The amount of residual dissolved peptide was determined using a Waters Alliance 2695 system equipped with an XBridge column (bridged ethylsiloxane/silica hybrid 130 C18 3.5 m to 4.6 50 mm) with a flow rate of 2 mL/min at 30 C and detection at 215/276 nm

The biosynthesis of glutathione occurs primarily in the cellular cytosol via a strictly regulated, two-step enzymatic pathway that requires adenosine triphosphate (ATP) for energy

Immunofluorescence Glass coverslips (thickness #1.5, diameter 12 mm, sterilized by baking overnight at 180 C) were pretreated with 10 g/mL fibronectin (Sigma, F1141) for 3 h at RT to ensure enhanced cell adhesion