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The supernatants were collected and the free PEG-SH concentration in them was determined using Ellmans assay, according to the manufacturers instructions

3.4 Other regulated cell death (RCD) pathways In addition to the three major RCD pathways discussed above, necroptosisa regulated form of necrotic cell death mediated by RIPK3-dependent phosphorylationhas emerged as another critical mechanism implicated in inflammatory diseases

doi:10.1016/j.biopsych.2008.04.022, PMID: 18534556 Magalhes PV, Dean OM, Bush AI, Copolov DL, Malhi GS, Kohlmann K, Jeavons S, Schapkaitz I, Anderson-Hunt M, Berk M (2011) N -acetylcysteine for major depressive episodes in bipolar disorder