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Carrier Oil : I use coconut oil, but you can also use almond oil, or jojoba oil for hydration

Thawing Media (NBM complete media containing 10 M Y-27632 ROCK inhibitor), Plating Media (NBM complete media containing 10 ng/mL BDNF, 10 ng/mL CNTF, 10 ng/mL GDNF, 10 M Y-27632 ROCK inhibitor, 1 B27, 5 g/mL puromycin, and 2 g/mL doxycycline hyclate), D7 Media (NBM complete media containing 10 ng/mL BDNF, 10 ng/mL CNTF, 10 ng/mL GDNF, 1 B27, 5 g/mL puromycin, and 2 g/mL doxycycline hyclate), and Maintenance Media (NBM complete media containing 10 ng/mL BDNF, 10 ng/mL CNTF, 10 ng/mL GDNF, and 1 B27) were prepared and equilibrated to 37 C immediately before use