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64 To investigate how the expression of PrP mutants impacted on Cu-mediated cell death, IF labelling of the mutant-expressing cells was combined with the Cell Event apoptotic assay

2 In pernicious anemia, the deficient levels of vitamin B12 are due to the body lacking the intrinsic factor that forms a complex with vitamin B12 which allows absorption into the gut

Its proven ability to bind and transport copper, influencing gene expression and cellular function, provides a robust foundation for groundbreaking discoveries

For this purpose, we made use of a mixed-species approach in which we diluted phosphopeptides enriched from yeast at different ratios into a fixed background of HeLa phosphopeptides and analyzed them by DDA, dDIA, and DIA (Fig