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Briefly, the proteins were blotted onto an activated PVDF membrane (0.2 m) in transfer buffer at 250 mA for 90 min and then blocked with 5% milk in Tris-buffered saline with Tween-20 (TBS-T)

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Moreover, recent studies have revealed a mechanistic link between the negative cell cycle regulator - retinoblastoma tumor suppressor (RB) and alternated glutathione homeostasis (Nicolay et al., 2013