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Further supporting this hypothesis, when A42 was added to synaptosomes isolated from brains of APOE targeted-replacement mice, the degree of lipid peroxidation assessed by protein-bound HNE and protein oxidation indexed by protein carbonyls and protein-resident 3-nitrotyrosine was greatest in synaptosomes from ApoE4 mice followed by ApoE3 mice and then ApoE2 mice (218), which is the reverse order of the number of Cys residues in the respective apoE proteins in brains of the respective ApoE mice

The scientific interest in this combination stems from the possibility of observing, in controlled experimental models, complementary biological pathways involved in cellular migration, stress response, actin dynamics, and structural repair processes

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The peroxidase was detected with ECL Western Blotting Reagent (Amersham)