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Homocysteine levels were inversely associated with muscle strength/physical function, total lean mass, and appendicular lean mass

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Amino acids analysis Concentrations of 25 amino acids (alanine, sarcosine, glycine, -aminobutyric acid, valine, -aminoisobutyric acid, leucine, allo- iso -leucine, iso -leucine, threonine, serine, proline, asparagine, aspartic acid, methionine, 4-hydroxyproline, phenylalanine, glutamic acid, -aminoadipic acid, cysteine, ornithine, lysine, histidine, tyrosine, tryptophan) were measured in the plasma samples by Trace 1,300 gas chromatograph (Thermo Scientific, Milan, Italy) coupled to an ITQ 700 ion trap mass spectrometer (Thermo Scientific, Austin, TX, USA)