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Description
Western Blot Western blotting was carried out as described previously [21,22], and brain homogenates were lysed on ice for 30 min in 100 mL of lysis buffer (120 mM NaCl, 40 mM Tris (pH 8), and 0.1% NP40) and centrifuged at 12,000 rpm for 30 min
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Answer: First effects (appetite suppression) are often noticeable within 3 to 5 days of the first injection
Other studies indicate the additional importance of the local microenvironment for the fate and maintenance of CSCs [242], especially those originating from mutated and transformed tissue-specific SPCs [243, 244]
