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These hallmarks represent the underlying biological mechanisms that contribute to the aging of living organisms

After homogenization, the supernatant was centrifuged and measured with a sensitive and specific ELISA according to the manufacturers instructions for TNF-, IL-10 and IL-1 (RayBiotech, Norcross, GA, USA)
Histamine induces peripheral and central hypersensitivity to bladder distension via the histamine H 1 receptor and TRPV1

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