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The next day, the spheroids were washed 3 times with SWB solution for 1 h each at 4 C and incubated with secondary antibodies conjugated with Alexa Fluor 488, TRITC, or Alexa Fluor 647 diluted 1:400 and DAPI (1:10,000) in SWB, overnight at 4 C on an orbital shaker
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