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doi: 10.5588/ijtld.12.0512

While other analogous and prodrugs have been proposed [41, 42], none have been tested clinically

While the saponified fraction extracted in petroleum ether was mixed with 50 L of bis(trimethylsilyl)trifluoroacetamide (BSTFA) + trimethylchlorosilane (TMCS) 99:1 sialylation reagent, and 50 L pyridine were used to derivatize sample functional groups to trimethylsilyl groups (abbreviated TMS) before GC analysis

Unless indicated, erythrocytes were cultured as previously described at 2% hematocrit in complete media (incomplete media supplemented with 27mM sodium bicarbonate, 11mM glucose, 5mM HEPES, 1mM sodium pyruvate, 0.37mM hypoxanthine, 0.01mM thymidine, 10g/mL gentamicin, and 0.5% Albumax I (Thermo-Fisher) under 5% O 2 /5% CO 2 /90% N 2 atmosphere at 37C ( Plasmodium falciparum Growth Inhibition Assays Asynchronous cultures diluted to 1% parasitemia in 100L wells were subjected to drug dilutions, solvent controls, and untreated uninfected erythrocyte controls in technical duplicates