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Membranes were blocked during 1 h at room temperature in 1 PBS containing 0.1% Igepal and 5% milk and then incubated overnight at 4C with the following primary antibodies: anti-CBS mouse monoclonal antibody (ab12476, Abcam, 1:1,000) and anti--tubulin mouse monoclonal antibody (T6793, Sigma-Aldrich, 1:10,000)
221,324,325 In a double-blind, placebo-controlled clinical trial, nanocurcumin with radiolabeled iodine treatment was associated with a lower frequency of increased micronuclei in lymphocytes versus the placebo group in patients with differentiated thyroid carcinoma

doi: 10.1016/j.nlm.2019.02.002 139 YangL.WuC.ParkerE.LiY.DongY.TuckerL.et al

Apart from that, ALT activity was measured in vascular and hepatic cell culture supernatants of liver models treated for up to 72 h
