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Description
Western blot analysis involved preparing whole cell lysates using RIPA lysis buffer, quantifying protein concentration via BCA assay, followed by SDS-PAGE, transfer to nitrocellulose membranes, incubation with primary and HRP-conjugated secondary antibodies, and detection using an ECL solution [1]
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Discussion In this work we revealed that FA alters the GSH:GSSG redox balance, triggers oxidative stress, and causes cytotoxicity in cells and organisms without any deficiency in DNA repair (Figs
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