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Sterile normal saline was purchased from Baxter (Deerfield, IL), RNeasy mini kits were purchased from Qiagen (Hilden, Germany), and all other chemicals were of analytical grade and purchased from Sigma-Aldrich (St.Louis, MO)

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Crucially, unlike apoptosis, which is typically immunologically silent, necroptosis results in cell lysis and the release of damage-associated molecular patterns, thereby provoking a potent inflammatory response (40)