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To simultaneously quantify the levels of persulfide and polysulfide sulfur, one aliquot of reaction mixture (100 L) was blocked with IAM-TPP (250 M) for procedure A and another aliquot of the same reaction (100 L) was blocked with IAM (250 M) for procedure B for 20 min at RT, protected from light

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QJM 105(5):445454 Anker SD et al (2009) Prevalence, incidence, and prognostic value of anaemia in patients after an acute myocardial infarction: data from the OPTIMAAL trial