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Revised Date: 21 May 2016

Pre-cursor to the antioxidant glutathione Due to the ability of thiols to undergo redox (reduction-oxidation) reactions, cysteine has antioxidant properties

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Results Circadian clock regulates GSH levels in fly heads We measured GSH levels in heads of wild type Canton S (CS) control flies collected at 4 h intervals around the clock and found significant oscillations with 1.5-fold amplitude such that the highest GSH concentrations were detected in the early morning at ZT 0 followed by a decline to a trough in midday at ZT 8 (Fig