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The ratio of GSH to GSSG is often used to gauge the level of oxidative stress in cells, with higher concentrations of GSSG implying more oxidative stress

Due to the high concentration of GSH in the cells, NO rapidly reacts with the cysteine thiol group of GSH to form S-nitrosoglutatione (GSNO)

As you age and are exposed to more metabolic stressors, you can move to 1000mg daily