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sgRNA expressing vector along with lentiviral packaging vectors Delta-VPR and CMV VSV-G were transfected into HEK-293T cells using the XTremeGene 9 transfection reagent (Roche)

After postdoctoral stints at the Drug Chemistry Center of Italian National Research Center, Italian National Institute of Health, University of Rome La Sapienza, and University of Siena, in 2001, he joined the Department of Pharmacy of the University of Salerno, where since 2016 he has been Full Professor in Medicinal Chemistry

Although we did not find any significant difference between soluble Flt-1, also known as vascular endothelial growth factor receptor 1, levels in the plasma of R and LC patients, LC group exhibits a significant plasma elevation compared to the HC cohort ( Figure 6I )