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Histological verification of cannula placement was accomplished by the injection of 5 fast-green dye via the guide cannula following the completion of behavioral testing

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Haem b was detected by monitoring the absorption at 400 nm

In the B12 injection kit: BD Emerald Syringes with with BD Microlance needles Alcohol Prep Pads saturated with 70% isopropyl alcohol for optimum anti-bacterial action B12 Injection Kit contains: 10 sterile syringes 2ml 10 sterile needles (21g green 1.5inch) for drawing the medication from the vial 10 sterile needles (25g orange 1inch) for injecting 10 pre-injection pads All items in the kit are sterile in individual blister packs and are EU & CE-certified