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Over time of 0.5 h, 4 h, and 22 h, the anti-ICAM NPs and pan-leukocyte marker (CD45) decreased in the lungs and increased up to 5-fold in the brain, suggesting the initial delivery of ICAM to activated leukocytes in the pulmonary vasculature followed by migration of leukocyte-loaded NPs to the injured brain across the BBB

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