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Importantly, commonly used in vitro BBB models, such as transwell systems and endothelial cell lines including RBE4, bEnd.3, and hCMEC/D3 monolayers, have important limitations, as they may overpredict permeability and do not fully recapitulate key in vivo BBB features, including physiological shear stress and multicellular neurovascular architecture (Chaulagain et al., 2023)

Consistent with western blotting results, GSTP1/P2 staining was greatly diminished at 48 h post-PH

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The pathogenicity of Th17 cells in autoimmune diseases