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10.1016/j.ymgme.2015.09.004 Mol

Half of the groups were subjected to a 16-day exercise regime, while the other half remained sedentary

If there are regularly scheduled breaks, and a lot of the focus is on drills that arent always at maximum intensity, it could be sufficient to eat small snacks during the breaks, in addition to copious hydration with water, instead of sports drinks

PCR was performed with a 2 l cDNA mixture in a total volume of 35 l using Taq polymerase and 0.2 nmol/l of each forward and reverse primer (intron spanning, 5CCCCCGATAGCTCCTACAAC3 and 3AAGGCCTTCCTCATGCACT5, TRPV1 mRNA accession number NM_018727) after initial 95 C for 3 min over 35 cycles of 95 C for 30 s, 64 C for 30 s and 72 C for 45 s, finalized by 72 C for 7 min and a temperature hold at 4 C in a Techne TC-512 gradient thermal cycler (Staffordshire, UK), yielding a 295 bp product