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Biotechniques 43 , 581572 (2007)
Melatonin elicits protective effects on OGD/Rinsulted H9c2 cells by activating PGC1/Nrf2 signaling
Then the pellets were stained for 20 min in 100 l assay buffer containing 5 g ml 1 propidium iodide (Sigma-Aldrich, P4170) and 3 l Annexin VFITC (BioLegend, 0640906)
