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The quantification of DNMT1, DNMT3a, and DNMT3b expression levels was performed using real-time PCR on an Applied Biosystems 7500 system

Aged RBCs exhibited a marked decrease in aminophospholipid translocase activity, an enzyme responsible for maintaining the asymmetric distribution of phospholipids across the cell membrane

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The size of the molecules selected may permit understanding of the different routes of transport, with molecules such as inulin, polyethylene glycols (PEGs) and Fluorescein Isothiocyanate-Dextran (FITC-Dextran) being utilised for assessment of the paracellular route, whilst larger molecules such as horseradish peroxidase can be utilised for assessment of the transcellular route [21]