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In some cases, these factors can even halt production completely

The synthesis of GSH from its constituent amino acids involves two ATP-requiring enzymatic steps: (1) the first step is rate-limiting and catalyzed by GCL which is composed of two subunits: one catalytic (GCLC) and one modifier (GCLM): (2) The second step is catalyzed by GSH synthetase (GS) [19]: Although GS is generally thought not to be important in the regulation of GSH synthesis, accumulating evidence suggests that GS may play an important role, at least in certain tissues and/or under stressful conditions [20]

Cells were imaged with an LFD-LSM880 Zeiss microscope equipped with a multi-line Argon laser and 405 nm diode laser with plan-Apochromat 63x/NA 1.4 oil DIC M27 immersion lens

* Delayed release to maintain stable amount throughout the day