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Description
We previously demonstrated that these doses and durations of treatment do not alter cortical neuron viability or increase oxidative stress (16)
LCMS metabolite quantification in HeLa cells A total of 1 10 6 treated HeLa cells or 1 10 6 Cas9-expressing HeLa cells were collected, washed with cold PBS and metabolic activity quenched by freezing samples in dry ice and ethanol, and stored at 80 C
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2.2 Eligibility criteria Studies were excluded if they met any of the following conditions: They were not original research (e.g., reviews, commentaries, or meta-analyses)
