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Intracellular melanin profile of HCMs The intracellular melanin histogram profile from the sample image field of HCMs was generated by determining the ratio fraction from each of the image segmented 2PM FLIM (and spectral) phasor clusters mapped to the heterogeneously pigmented intracellular melanin

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therefore, the examination of all lesions is an essential step in the recognition process because it allows the identification of lesions deviating from the individuals prevailing benign pattern (concept of the ugly duckling sign) [4245] (Fig