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A 2018 study in the Journal of the American Heart Association found that higher glutathione levels were associated with a lower risk of cardiovascular disease in a large cohort study (Shimizu et al., 2018)

To overcome this issue, we employed the Ub-R54A mutant (Ohtake et al, 2016), which prevents cleavage at the R54 residue and generates the peptides containing GlyGly-modification at both K48 and K63 residues (Fig

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