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* Correspondence: Michele Ghidini, [email protected] Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

After 15 min agitation at 8 C followed by 15 min centrifugation at 20800 g and 4 C, the supernatant was collected in a 15 ml Falcon tube and protein content was measured using the DC TM Protein Assay (Biorad, Feldkirchen, Germany)