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Methods Cell lines and reagents HEK293T and HeLa cells were maintained in high-glucose DMEM (Sigma-Aldrich, D6429) containing 10% fetal calf serum (Sigma-Aldrich, P4333), 100 U ml 1 penicillin and 100 g ml 1 streptomycin at 37 C and 5% CO 2

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Plasma levels of -endorphin, leucine enkephalin and arginine vasopressin in patients with essential hypertension and the effects of clonidine

Visualisation of inhibition of bacterial invasion of bladder epithelial cells To provide visual support to the invasion assay above, our clinical model strain for P