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The activation of ULK1 kinase complexes, which facilitates the binding of the autophagy-associated protein 8 ATG8 protein family (also known as LC3s and GABARAPs) to phosphatidylethanolamines or phosphatidylserines and anchors them to autophagic membranes, is what initiates autophagy[109]

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Band intensities corresponding to 3-AR were normalized to -actin expression using MCID imaging software