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Draw the intended total-blend dose for your vial size, then check for air bubbles and push any out

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We analyzed three different endogenous controls, Gadph, Actb, and Rn18s , and several genes of interest, Vegfr2, Src, Nos2, Nos1, Nos3, Akt1, Kras, Mapk1, Srf, Foxo1, Nfkb1, and Egr1