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Reactions were performed with 5 M histone H3 (histone L-arginine) substrate, 1 M radiolabeled S-adenosyl-L-[methyl- 3 H] methionine (SAM) co-substrate, and recombinant human PRMT3 enzyme

Alternate Sites: If rotating injection sites to prevent lipohypertrophy (tissue buildup), you may also use the outer thigh or upper arm fatty tissue
William Russell (Director, Mass Spectrometry Facility, Department of Biochemistry and Molecular Biology, UTMB) for his assistance in optimizing and running all LC/MS/MS assessments
