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3T3 fibroblasts were maintained in culture as described previously [24] and plated in 2 cm 2 LAB-TEK II chambered cover glass (Nunc, Thermo Fischer Scientific, Waltham, MA, USA) for 5 days, 72 h, 48 h, 24 h, and 6 h before the experiment and treated after attaching as explained before

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Needed for bone growth and bone remodeling by osteoblasts and osteoclasts Prevention of rickets in children and osteomalacia in adults Together with calcium, vitamin D also helps protect older adults from osteoporosis