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They found that a composition ionizable lipid: DSPC: DOPE: Cholesterol: C14-PEG at 38.8:3.6:10.9:44.5:2.25 mol%, which was in between the two tested extreme conditions of i) ionizable lipid: DSPC: DOPE: Cholesterol: C14-PEG at 35:0:16:46.5:2.5 mol% and ii) ionizable lipid: DSPC: DOPE: Cholesterol: C14-PEG 50:10:38.5:0:1.5 mol%, was the optimal for all tested applications, such as silencing, gene expression, size and entrapment

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