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Next, the sections were immersed in Weigert's iron hematoxylin staining solution for 1 min, followed by staining in Van Gieson's acid fuchsin for 6 min at 25C
It is also notable that free zinc deficiency contributes to the inhibition of the N -methyl-d-aspartate receptor (NMDAR), which leads to an increased level of ROS [35, 58,59,60]
doi: 10.1210/jendso/bvz039 56
These radicals can further damage mitochondrial DNA, proteins, and lipids, impairing mitochondrial function and reducing ATP production, which is essential for cardiac muscle contraction [87]
