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J Feline Med Surg 2011;13:467-472
R red and R ox represent the fluorescence ratios of fully reduced and fully oxidized roGFP, respectively
Here, the inhibitory effect of ENDO compounds on the binding of Munc13-4 to STX7 was confirmed by an orthogonal validation assay, consisting of the pulldown of Munc13-4 with recombinant STX7
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