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Differences in steroid 5 alpha-reductase iso-enzymes expression between normal and pathological human prostate tissue

Sections were then washed three times before being incubated with secondary antibodies (1:400, species-specific Alexa Flour 488, 555 or 647, Thermo Fisher Scientific), diluted in PBS for 2 h at room temperature on a shaker at 40 rpm

The higher levels of exon deleted variants of NQO1 represents a potential explanation for the decreased enzymatic activity of cells carrying the NQO1 * 3 polymorphism (Lienhart et al., Post-translational modifications of NQO1 Post-translational modifications (PTM) of NQO1 including phosphorylation, ubiqitination and acetylation have been detected in large proteomic studies

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