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Our observation that intracellular NA concentrations did not increase following NNMT inhibition extends previous studies that showed NA concentrations were largely unaffected following NNMT knockdown with either adenovirus[39] or anti-sense oligonucleotides (ASOs).[12] It is possible that significant accumulation of NA in adipocytes (and other tissues) is prevented by rapid conversion of NA to NMN and subsequent NAD + synthesis or transport to the extracellular environment.[27] Importantly, NNMT inhibition potentiated the absolute intracellular concentrations of NAD + and SAM, without altering the levels of NA and SAH in the adipocytes

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Another study comparing groups of severely obese versus non-obese patients showed that 34.1% of people in the obesity group had vitamin B12 deficiency, compared with 12.5% in the control (non-obese) group

Research suggests that AOD-9604 may activate beta-3 adrenergic receptors in fat cells, leading to enhanced fat oxidation