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It is also important to consider that excessive folic acid supplementation may have unintended biological effects

examples of suitable fluorescent materials include umbelliferone, fluorescein, fluorescein isothiocyanate, rhodamine, dichlorotriazinylamine fluorescein, dansyl chloride or phycoerythrin

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The absorbance of the cells in the wells was then measured at 490 nm using the Multiskan Sky Microplate Spectrophotometer (Thermo Fisher Scientific, Inc.) to evaluate the cell viability