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Contact with the bone usually indicates the needle contact with the wings of the sacrum or the iliac bone, superior to and near the greater sciatic notch

[DOI] [PMC free article] [PubMed] [Google Scholar] 52.Netea M.G., Dominguez-Andres J., Barreiro L.B., Chavakis T., Divangahi M., Fuchs E., Joosten L.A.B., van der Meer J.W.M., Mhlanga M.M., Mulder W.J.M., et al

Reduction and alkylation of the cysteine residues were then performed with 10 mM DTT and 55 mM iodoacetamide, respectively, followed by adding 360 L of 50 mM ammonium bicarbonate buffer to reduce the urea concentration to below 1 M