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For the perfusions, mice were deeply anesthetized with isoflurane and perfused transcardially with saline, followed by a 10% buffered formalin solution (150200 mL per mouse)
Xu, M., Wang, Y., Xia, R., Wei, Y
Meanwhile, Nef promotes neuropathology by upregulating CCL2 expression in microglia and augmenting IL-6 and IL-8 production in astrocytes via the PI3K/Akt/PKC, p38 MAPK, NF-B, C/EBP, and AP-1 signaling pathways [137,138,139]
This is potentially due to the sample containing a sub-fraction of mis-folded protein or the samples being partially refolded
