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Tissue metabolite concentrations were calculated as follows: where vol estimated is the estimated total volume of the polar layer, vol collected is the volume of polar layer collected, and units are expressed as nmol/g

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To ease access to this resource, we made all the data sets and statistical analyses available via a web application with an interactive interface ( While temporal RNA-Seq data from control mice and mice deficient for circadian clock genes (total or kidney specific) have already been published (9, 48), temporal high-throughput proteomics analyses have been limited to the WT mouse liver (28, 49, 50), macrophages (30), SCN (31), and forebrain synapse (51)