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For the orthotopic pancreatic cancer model, VRK2-WT or VRK2-KO Pan02 cells in logarithmic phase (8090% confluence) were harvested, detached with 0.25% trypsin-EDTA, centrifuged (1000 rpm, 5 min), and resuspended in cold serum-free RPMI-1640 medium

Lyophilization, or freeze-drying, is a process that removes water from the peptide, transforming it into a stable, powdery cake that can be shipped and stored at room temperature for extended periods without degrading

Overproduction of ROS leads to the translocation of NF-B to the cell nucleus and provides cytokine, chemokine, and growth factor genes with access to promoter regions