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mansoni TGR was amplified by PCR (forward primer 5-CTATTTGGCTAGACGTCTGT-3 and reverse primer 5-AATACAGTTTCCTTCCCGTT-3) and cloned into PCRII-TOPO vector (Invitrogen, The resulting construct was linearized using XhoI (for SP6 RNA polymerase transcription) and SacI (for T7 RNA polymerase transcription)

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35 The segment of BMP that interacts with BMP receptors (AISMLYLDENEKVVL) is derived from amino acid residues 91105 found in the mature human BMP-2 protein

The statistical test(s) used in each experiment is listed in the figure legends