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Cur loading into unloaded LCS sporopollenin microcapsules LCS sporopollenin isolation from raw pollens The suspension of raw Lycopodium clavatum pollens was carried out in acetone, followed by refluxing at 50 C with gentle stirring for a duration of 3 h

Quantification of the amount of antibodies against PVL in the plasma Antibodies against PVL in the plasma were measured with a specific ELISA method with solid-phase LukF-PV and LukS-PV using a protocol adapted from Croze et al 23

The remaining O2 flux after inhibition with antimycin A (O2 flux independent of the electron transfer system) was measured