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The Seahorse buffer for cells cultured in glucose-deprived medium consisted of glucose-free DMEM, phenol red, 2 mM sodium pyruvate, and 2 mM glutamine
Mitra S, Nguyen LN, Akter M, Park G, Choi EH, Kaushik NK (2019) Impact of ROS generated by chemical, physical, and plasma techniques on cancer attenuation
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To do this, you will employ three different wells, one with Sample, one with Sample plus Internal Standard, and one that will be the Sample Blank
