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The total dilution of the reaction mixture I was as follows: (124/104) (1277/108) = 14.09793: this was approximately equal to the millimolar absorption coefficient of the reaction product of GSH with Ellmans reagent

The timing of binders is critical

NedelcovychMTKimBHZhuXLovellLEManningAAKelschenbachJet alGlutamine antagonist JHU083 normalizes aberrant glutamate production and cognitive deficits in the EcoHIV murine model of HIV-associated neurocognitive disorders
